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Retina Organoid Protocol References

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Retina Organoid Protocol References

This is the start of a list of references that describe methods for generating retina organoids. Please add to the list via comment. 

Self-organizing optic-cup morphogenesis in three-dimensional culture
http://www.ncbi.nlm.nih.gov/pubmed/21475194 
Eiraku M, Takata N, Ishibashi H, Kawada M, Sakakura E, Okuda S, Sekiguchi K, Adachi T, Sasai Y.
Nature. 2011 Apr 7;472(7341):51-6. doi: 10.1038/nature09941.

Self-formation of optic cups and storable stratified neural retina from human ESCs
https://www.ncbi.nlm.nih.gov/pubmed/22704518
Nakano T, Ando S, Takata N, Kawada M, Muguruma K, Sekiguchi K, Saito K, Yonemura S, Eiraku M, Sasai Y.
Cell Stem Cell. 2012 Jun 14;10(6):771-85. doi: 10.1016/j.stem.2012.05.009.

Generation of three-dimensional retinal tissue with functional photoreceptors from human iPSCs
http://www.ncbi.nlm.nih.gov/pubmed/24915161 
Zhong X, Gutierrez C, Xue T, Hampton C, Vergara MN, Cao LH, Peters A, Park TS, Zambidis ET, Meyer JS, Gamm DM, Yau KW, Canto-Soler MV.
Nat Commun. 2014 Jun 10;5:4047. doi: 10.1038/ncomms5047.

Generation of highly enriched populations of optic vesicle-like retinal cells from human pluripotent stem cells
https://www.ncbi.nlm.nih.gov/pubmed/25640818
Ohlemacher SK, Iglesias CL, Sridhar A, Gamm DM, Meyer JS.
Curr Protoc Stem Cell Biol. 2015 Feb 2;32:1H.8.1-20. doi: 10.1002/9780470151808.sc01h08s32.

Retinal organoids from pluripotent stem cells efficiently recapitulate retinogenesis
https://www.ncbi.nlm.nih.gov/pubmed/27050948 
Völkner M, Zschätzsch M, Rostovskaya M, Overall RW, Busskamp V, Anastassiadis K, Karl MO.
Stem Cell Reports. 2016 Apr 12;6(4):525-38. doi: 10.1016/j.stemcr.2016.03.001. 

Reprogramming of mouse retinal neurons and standardized quantification of their differentiation in 3D retinal cultures
https://www.ncbi.nlm.nih.gov/pubmed/27658012  
Hiler DJ, Barabas ME, Griffiths LM, Dyer MA.
Nature Protocols. 2016 Oct;11(10):1955-1976.  doi: 10.1038/nprot.2016.109. 

 

Our recent protocol

Hi Jessica,

You may consider our protocol that was published in Stem Cell Reports recently:

Intercellular Adhesion-Dependent Cell Survival and ROCK-Regulated Actomyosin-Driven Forces Mediate Self-Formation of a Retinal Organoid.

https://www.ncbi.nlm.nih.gov/pubmed/27132890 

Stem Cell Reports. 2016 May 10;6(5):743-56. doi: 10.1016/j.stemcr.2016.03.011. Epub 2016 Apr 28.

Lowe A1Harris R1Bhansali P1Cvekl A2Liu W3.

 

Wei

Re: Our recent protocol

Thank you Wei. I've formatted your entry and added the PubMed link so others can easily access the manuscript.