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Retina Organoid Protocol References

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Retina Organoid Protocol References

This is the start of a list of references that describe methods for generating retina organoids. Please add to the list via comment. 

Self-organizing optic-cup morphogenesis in three-dimensional culture 
Eiraku M, Takata N, Ishibashi H, Kawada M, Sakakura E, Okuda S, Sekiguchi K, Adachi T, Sasai Y.
Nature. 2011 Apr 7;472(7341):51-6. doi: 10.1038/nature09941.

Self-formation of optic cups and storable stratified neural retina from human ESCs
Nakano T, Ando S, Takata N, Kawada M, Muguruma K, Sekiguchi K, Saito K, Yonemura S, Eiraku M, Sasai Y.
Cell Stem Cell. 2012 Jun 14;10(6):771-85. doi: 10.1016/j.stem.2012.05.009.

Generation of three-dimensional retinal tissue with functional photoreceptors from human iPSCs 
Zhong X, Gutierrez C, Xue T, Hampton C, Vergara MN, Cao LH, Peters A, Park TS, Zambidis ET, Meyer JS, Gamm DM, Yau KW, Canto-Soler MV.
Nat Commun. 2014 Jun 10;5:4047. doi: 10.1038/ncomms5047.

Generation of highly enriched populations of optic vesicle-like retinal cells from human pluripotent stem cells
Ohlemacher SK, Iglesias CL, Sridhar A, Gamm DM, Meyer JS.
Curr Protoc Stem Cell Biol. 2015 Feb 2;32:1H.8.1-20. doi: 10.1002/9780470151808.sc01h08s32.

Retinal organoids from pluripotent stem cells efficiently recapitulate retinogenesis 
Völkner M, Zschätzsch M, Rostovskaya M, Overall RW, Busskamp V, Anastassiadis K, Karl MO.
Stem Cell Reports. 2016 Apr 12;6(4):525-38. doi: 10.1016/j.stemcr.2016.03.001. 

Reprogramming of mouse retinal neurons and standardized quantification of their differentiation in 3D retinal cultures  
Hiler DJ, Barabas ME, Griffiths LM, Dyer MA.
Nature Protocols. 2016 Oct;11(10):1955-1976.  doi: 10.1038/nprot.2016.109. 


Our recent protocol

Hi Jessica,

You may consider our protocol that was published in Stem Cell Reports recently:

Intercellular Adhesion-Dependent Cell Survival and ROCK-Regulated Actomyosin-Driven Forces Mediate Self-Formation of a Retinal Organoid. 

Stem Cell Reports. 2016 May 10;6(5):743-56. doi: 10.1016/j.stemcr.2016.03.011. Epub 2016 Apr 28.

Lowe A1Harris R1Bhansali P1Cvekl A2Liu W3.



Re: Our recent protocol

Thank you Wei. I've formatted your entry and added the PubMed link so others can easily access the manuscript.